This paper applied quantitative grain fluorescence techniques to analyzing the QGF-E and QGF property of 101 rock samples from 5 wells in tight sandstone and thin siltstone in the third section of the Shahejie Formation in the Niuzhuang sag of the Dongying depression. It was observed that both the tight sandstone and thin siltstone show generally high fluorescence abundance of hydrocarbon and therefore suggests that they are possible subtle oil-migration pathways in the present or geological time. These thin subtle oil-migration pathways afforded important clues for the research of hydrocarbon accumulation in lithological pools in the middle and lower of Es3 in deep sag zones which has the hydrocarbon source from the upper of Es4 when there is no apparent fault playing oil migration conduits to connect lithologic traps and deep source rocks. This study shows good prospect of QGF techniques in discriminating of oil migration pathways and paleo-oil layer. This study results may have important significance in researching of hydrocarbon accumulation mechanism of subtle reservoirs in the Dongying depression and other areas.