黑潮源区沉积物古菌多样性调查及研究
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中国科学院知识创新项目


Diversity of Archaea in the Sediments from the Source Area of Kuroshio
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    摘要:

    为了研究西太平洋黑潮源区不同深度沉积物中古菌群落结构的多样性,应用16S rDNA文库技术,对IMAGES XIV航次采集的岩芯MD063047进行基因文库的构建和分析,得到260个克隆,经分析处理后得到56个OTUs(Operational Taxonomic Units)。基于16S rRNA序列的同源性比较,绘制系统进化树,进行统计学分析。研究结果显示,西太平洋黑潮源区的本哈姆高原(Benham Rise)沉积物中有丰富多样的古菌群落,在1m内的沉积物中分布着9种古菌类群。古菌序列归属于泉古菌(Crenarchaeota)和广古菌(Euryarchaeota),并以泉古菌为主(占总序列的91.5%)。其中,泉古菌包含MGI、MBGB、MCG和UCIIb 4个类群,并且主要由MGI构成(占总序列的40.8%),而广古菌包含MBGD、SAGMEG、DHVEG、MBGE和VAL III 5个类群,各类群所占总序列比例均较低(占总序列的0.4%~5.4%之间)。研究结果对于理解西太平洋黑潮源区沉积物中古菌多样性有重要意义,并为今后研究古菌群落与环境之间的关系提供科学依据。

    Abstract:

    To study the archaeal diversity of deepsea sediments from the Source Area of Kuroshio in Western Pacific, the MD063047 sediment core collected by the IMAGES XIV cruise was used to built a genetic base through 16S rDNAanalysis.260 archaeal 16S rDNA clones were obtained and after examination, a total of 56 OTUs were yielded. Phylogenetic results show that the sediments from Benham Rise contain a variety of thearchaea and nine groups have been identified within 1 meter of sediments. Archaea belongs to Crenarchaeota and Euryarchaeota, with Crenarchaeota accounting for 91.5%of total archaeal clones. Crenarchaeota consists of four groups (MGI, MBGB, MCG and UCIIb), and is dominated by MGI (40.8% of total archaeal clones). And Euryarchaeota containsfive archaealphylotypes, which all take up tiny percentage (0.4%~5.4%) of total archaeal clones.The results have important implications for understanding of archaeal community in deepsea sediments from the source area of Kuroshio in Western Pacific and provide a scientific basis for the future study on the relationship between archaea and environment.

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景天爽,于心科.2012.黑潮源区沉积物古菌多样性调查及研究[J].地质学报,86(12):2011-2019.
Jing Tianshuang, Yu Xinke.2012. Diversity of Archaea in the Sediments from the Source Area of Kuroshio[J]. Acta Geologica Sinica,86(12):2011-2019.

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历史
  • 收稿日期:2012-03-02
  • 最后修改日期:2012-04-13
  • 录用日期:2012-04-17
  • 在线发布日期: 2012-12-18