The noble gas contains six elements He, Ne, Ar, Kr, Xe, Rn, because of its chemical properties is very stable, generally do not participate in chemical reaction process. The noble gas in different earth spheres and extraterrestrial materials has a huge different abundance and isotope characteristic, and is one important tracer in earth science researches. Analytical techniques of noble gas isotopic composition has three methods for the gas collection, i.e. stepped heating, in vacuo crushing and laser micro zone ablation. The obtained gases will go through multi-stage purify system, and finally are tested by static noble gas isotope mass spectrometry. As the sensitive tracer, noble gas isotope can be used to identify the source of ore-forming materials and fluids, especially for the mantle original contribution, crust-mantle interaction and metallogenic relationship, in economic geological researches. It can be used to trace and reveal the differences among the ore-forming stages and the evolution of fluids and mineralizations. In addition, noble gas isotope also can indicate the behavior of fluid in ore-forming processes. Noble gas isotope provides an important scientific research tool for detailed mineralization study, metallogenic difference research and large-scale mineralization exploring.