The Luyuan epithermal gold deposit is located at the Qiongheba ore cluster area in the eastern segment of YemaquanQiongheba Paleozoic arc belt, eastern Junggar. Orehosting strata contain one suite of intermediatefelsic volcanic lava intercalated with volcaniclastic rocks, with orebodies occuring as veins, banding, lentoid and controlled by faults. Four stages of hydrothermal mineralization were identified, which are quartzpyrite stage (S1), quartzAupyrite stage (S2), quartzAupolymetallic sulfide stage (S3) and quartzcarbonate stage (S4), with S2 and S3 as the main Au mineralization stages. In this paper, the authors carried out detailed study of sulfur and lead isotopic compositions and rare earth elements of the sulfide from the deposit so as to reveal the oreforming material source. The results demonstrate that the sulfides have a narrow δ34S range of ＋0.2‰ to ＋2.8‰, with an average of ＋1.35‰, suggesting that the sulfur is characteristic of magmatic sulfur. The δ34S values of sulfides in ores and country rocks are in agreement, indicating that sulfur mainly derived from the Batamayineishan Formation of Upper Carboniferous. The 206Pb/204Pb, 207Pb/204Pb and 208Pb/204Pb ratios of sulfide from S2 and S3 are 16.457~18.084, 15.267~15.635 and 36.472~38.379, respectively. The 206Pb/204Pb, 207Pb/204Pb and 208Pb/204Pb values of one feldspar sample are 18.546, 15.509 and 38.183, respectively. The μ values vary from 9.11 to 9.58, while the ω values vary from 33.97 to 38.61. The Pb isotopic ratios vary greatly, far larger the changing range of normal Pb isotopic compositions. Isotopic compositions and feature parameters suggest that the anomalous lead of the Luyuan deposit should have a mixing origin, with part from the upper crustal material and the other from the mantle material. REE geochemical characteristics of sulfides are similar to those of the ore bearing volcanic rocks, implying that the ore forming materials likely came from ore bearing volcanic rocks. The hydrogen and oxygen isotopic compositions indicate that the ore forming fluid of the Luyuan gold deposit mainly sourced from meteoric water with minor magmatic water. Finally, combined with S and Pb isotopic and REE analysis, the study suggest that the magmatic activities and mineralization of the Luyuan mining area are characterized by crust mantle mixing and ore forming materials by deep source, more directly from ore hosting volcanic rocks.