The Cretaceous- Paleogene interval witnessed the most important events including the extinction of dinosaurs and other organisms and the rise of mammals. Therefore, the Cretaceous- Paleogene boundary (KPB) has received highly concerns worldwide. In order to find a more continuous and more typical non- marine KPB profile, extensive geological survey, profiling, scientific drilling, systematic sample collection and chemical analysis were carried out in the Pingyi Basin, Shandong province. Three typical sections of carbonate lacustrine facies with KPB were selected. Based in the identification of a large number of fossils, one charophyte assemblage of “ Festiella anluensis- Microchara cristata- Chara yuntaishanensis var. acuta ”, one ostracod fauna of “ Talicypridea reticulate- Mongolocypris distribute- Ziziphocypris simakovi- Frambocythere tumiensis ferreri ” and one sporopollen assemblage of “ Ulmipollenites+Ulmoideipites- Rugubivesiculites- Schizaeoisporites ” are established in the lower part of Member I of Dianqiao Formation. Besides, one charophyte assemblage of “ Peckichara zhijiangensis- Gyrogona wubaoensis- Stephanochara huangjianensis- Peckichara varians ”, one ostracod fauna of “ Candona biaoqiaoensis- Cyclocypris changleensis- Cypridea ” and one sporopollen assemblage of “ Deltoidospora- Ephedripites- Parcisporites parvisaccus- Ulmipollenites minor ” are established in the upper part of Member I of Dianqiao Formation. According to the characteristics of fossil assemblages, the KPB should be placed in the first member (Member I) of the Bianqiao Formation. This study clarified the Late Cretaceous- Paleocene biological assemblages in the Pingyi Basin, which is of great importance for studying the evolution law of biota near K/Pg and restore the evolution law of paleoclimate, paleogeography, paleoecology and carbonate lake basin in the Pingyi Basin.