Abstract:Although the Carboniferous is one of the most significant gas- source rocks in the Junggar basin, its oil- generating potential remains unclear. This study integrated geochemical analysis (stable carbon isotopes and biomarker fingerprints), basin modeling, structural evolution restoration, hydrocarbon generation simulation, and well- seismic joint tracing to elucidate the contribution of Carboniferous source rocks to oil reserves in the southeastern Junggar basin. We reconstructed the petroleum system evolution and delineated the spatial extent of effective source kitchens. Our results identified two types of crude oil in the southeastern Junggar basin. Type I oil exhibits relatively heavy stable carbon isotopes, a high Pr/Ph ratio, abundant C24 tetracyclic terpanes and C31 hopanes, barren tricyclic terpanes, gammacerane, β- carotane, and rearranged steranes. These characteristics correlated strongly with the Upper Carboniferous Batamayineishan source rocks, suggesting deposition in a fresh, oxygenated environment with organic input mainly from higher plants. In contrast, Type II oil displays geochemical features opposite Type I, reflecting a saline, reducing environment with input mainly from bacteria and algae. This oil type correlates well with the middle Permian Lucaogou source rocks. During the Middle- Late Jurassic, the Batamayineishan source rocks reached the oil- generating peak, forming two types of oil reservoirs: “self- generated and self- stored” and “lower- generated and upper- stored.” The widespread distribution and significant thickness of the Batamayineishan source rocks resulted in an area reaching 2031 km2 with an oil- generating intensity greater than 4×106 t/km2. Two oil- generating centers with intensities exceeding 6×106 t/km2 were discovered: the eastern slope of the Fukang sag and the Jimsar sag. These findings confirm the substantial oil- generating capacity of Carboniferous source rocks in the Junggar basin and highlight a new exploration area for the Carboniferous petroleum system in the southeastern Junggar basin.