Carry out research about the method of analyzing the Helium isotope composition of the inclusions of mineral with the Helix SFT noble gas mass spectrometer, Build the crusher and purification system for the sample, and measure the background of the whole system. Discuss the efficiency of the multiplier of the instrument, for the precise measurement of the 3He.Determine the calibration factor of the mass discrimination of the Helium isotope measurement through the measurement of the Helium in air. Through the measurement of the Helium isotope in the inclusion of the pyrite samples，obtain high reproducible isotopic ratio data, which precision reaches as high as 99%.